Novogene’s amplicon sequencing services play a pivotal role in Next-Generation Sequencing, offering precise microbial identification through key target genes like 16S rRNA. From DNA extraction to library preparation, the process delves into microbial diversity across environments. Its applications span environmental, agricultural, and health sciences, providing vital insights while supported by educational resources and advanced technology. The comprehensive sequencing methodologies extend capabilities in research innovation.
Understanding Novogene Amplicon Sequencing
Novogene’s amplicon sequencing services are a cornerstone in the field of Next-Generation Sequencing (NGS), renowned for their precision in identifying and distinguishing microbial species across various environments. This sequencing method concentrates on target genes such as 16S rRNA, 18S rRNA, and Internal Transcribed Spacer (ITS), which are vital for phylogeny and taxonomy studies of bacteria and fungi in these areas of research.
The Metagenomic Sequencing Process
Novogene’s workflow for amplicon metagenomic sequencing begins with DNA extraction, followed by PCR amplification, purification, quantification, pooling, and library preparation. The company offers a comprehensive guide on how specific hypervariable regions of conserved genes are amplified and sequenced using advanced NGS technology. This process is crucial for exploring microbial diversity and community compositions in complex environments.
Applications in Research
Amplicon sequencing is extensively applied in numerous research fields, such as environmental science, agriculture, and health sciences. This technology enables the identification of single microbial species in cultures and allows researchers to characterize and compare species diversity across different sources. This capability is particularly beneficial in microbiota studies of animals or plants and in assessing microbial community structures.
Benefits of Amplicon Sequencing
The use of universal primers targeting key genes allows amplicon sequencing to deliver high-resolution insights into microbial communities. Novogene’s utilization of Illumina NovaSeq 6000 ensures high coverage and flexibility in data analysis, with services like alpha- and beta-diversity analyses and taxonomy annotation. These advanced methodologies enhance the efficiency of research, enabling the resequencing of even GC-rich regions without host contamination.
Comparing Sequencing Technologies
While amplicon sequencing provides detailed insights into target-specific microbial regions, shotgun metagenomic sequencing offers a broader view by encompassing entire genomic DNA from samples. This approach yields both taxonomic and functional profiles, unlocking genetic information from microorganisms that cannot be cultured in labs, thus providing a comprehensive perspective on microbial communities.
Educational Support and Resources
Novogene’s dedication to education is evident in their range of resources, including webinars and expert insights. They guide researchers through metagenomic sequencing strategies and applications, enhancing understanding and optimization of metagenomic analysis. This support helps researchers tailor their projects to specific goals and to make the best use of genomic technologies.
Why You Should Learn More About Novogene Amplicon Sequencing Today
Understanding the intricacies of Novogene’s amplicon sequencing offers significant advantages to researchers seeking to explore complex microbial environments. It delivers precise, targeted insights while providing comprehensive support and educational resources. With potential applications in various research fields, from environmental science to health-related studies, this technology is an indispensable tool for modern genetic analysis. Engaging with Novogene’s innovative sequencing services equips researchers with cutting-edge analytical capabilities that can drive scientific discovery and innovation.